Extracellular expression and antiviral activity of a bovine interferon-alpha through codon optimization in Pichia pastoris
نویسندگان
چکیده
منابع مشابه
Heterologous Expression of Bovine Prochymosin in Pichia pastoris GS115
Objectives: In present research we evaluate the expression of this critical enzyme in a eukaryotic system for future use in cheese industry. Materials and Methods: We have cloned bovine prochymosin gene in methylotrophic yeast, P. pastoris, using pPIC9K as an expression vector. The recombinant plasmid was transformed into the host by electroporation, and it was expre...
متن کاملCodon Optimization Significantly Improves the Expression Level of a Keratinase Gene in Pichia pastoris
The main keratinase (kerA) gene from the Bacillus licheniformis S90 was optimized by two codon optimization strategies and expressed in Pichia pastoris in order to improve the enzyme production compared to the preparations with the native kerA gene. The results showed that the corresponding mutations (synonymous codons) according to the codon bias in Pichia pastoris were successfully introduced...
متن کاملEffect of codon optimization on expression levels of human cystatin C in Pichia pastoris.
Human cystatin C (CysC) is a cysteine proteinase inhibitor with many potential applications. To facilitate further studies of the functions and applications of CysC, we improved the heterologous expression of CysC using a basic codon optimization method. In this study, we cloned the high-GC content wild-type sequence of the CysC gene and also designed a slightly AT-biased sequence, with codons ...
متن کاملP-197: Expression of Bovine Follicle Stimulating Hormone Subunits in Pichia Pastoris
Background: Bovine follicle stimulating hormone (bFSH) belongs to the family of glycoprotein hormones that are released from the pituitary gland or the placenta. This hormone is a heterodimer consisting of common α- and specific β-subunit. Bovine FSH is used for reproductive technology such as superovulation in farm animals and polycystic ovary syndrome treatment. Also this hormone is produced ...
متن کاملheterologous expression of bovine prochymosin in pichia pastoris gs115
objectives: in present research we evaluate the expression of this critical enzyme in a eukaryotic system for future use in cheese industry. materials and methods: we have cloned bovine prochymosin gene in methylotrophic yeast, p. pastoris, using ppic9k as an expression vector. the recombinant plasmid was transformed into the host by electroporation, and it was expressed in optimum conditions...
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ژورنال
عنوان ژورنال: Microbiological Research
سال: 2016
ISSN: 0944-5013
DOI: 10.1016/j.micres.2016.05.009